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This therapeutic approach can be employed either as a standalone treatment or in combination with other therapies to inhibit tumor progression ( Herpes simplex virus type 1 (HSV-1) possesses a genome consisting of 152 kb of double-stranded linear DNA that encodes approximately 85 protein-coding genes, with 47 being dispensable in cell culture ( Currently, there are two primary directions for genetic modification of oncolytic HSV-1 ( Figure 1 )

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